A new study from NAI's Montana State University Team appears in the current issue of the Journal of the American Chemical Society. The study probes the hydrogenase enzyme, a large, complex enzyme which plays a major role in anaerobic metabolism by creating molecular hydrogen. The research team produced a crystal structure of the enzyme to unprecedented resolution, revealing a new level of detail in the enzyme's active site, and providing clues about it's evolution. These results further our understanding of the transition from the abiotic (non-living) world to the biological world which may have been an early event in the development of life on Earth, and possibly a common feature of life elsewhere in the universe. [Source: NAI Newsletter]

Posted by Keith Cowing at 3:24 PM | Permalink | Comments (0)

The National Aeronautics and Space Administration (NASA) Headquarters has released NASA Research Announcement (NRA) NNH08ZTT003N entitled "Research Opportunities for Fundamental Space Biology Investigations in Microbial, Plant and Cell Biology". The full text of the solicitation is available on the NASA Research Opportunities homepage at http://nspires.nasaprs.com under menu listing "Open Solicitations".

Continue reading "NASA Research Announcement (NRA) NNH08ZTT003N "Research Opportunities for Fundamental Space Biology Investigations in Microbial, Plant and Cell Biology"" »

Posted by Keith Cowing at 12:40 PM | Permalink | Comments (0)

Dear Colleague, The National Science Foundation's Assembling the Tree of Life (AToL) solicitation has recently been renewed and updated (see the program solicitation, NSF 08-515; http://www.nsf.gov/publications/pub_summ.jsp?ods_key=nsf08515). As in the past, the AToL competition will support creative and innovative research to resolve evolutionary relationships for large groups of organisms. The program also supports research on theory and methods and tool development for these large scale phylogenetic investigations. With this letter we wish to draw your attention to several new and/or enhanced areas of interest. Proposals in the following areas are especially encouraged:

Continue reading "NSF Dear Colleague Letter - Assembling the Tree of Life Solicitation" »

Posted by Keith Cowing at 11:25 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 819-823

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2007.0207

A key challenge in Astrobiology is to comprehend life and its interaction with the environment at a level sufficiently fundamental to embrace the alternative biochemistries that may be encountered in a search for life elsewhere (Baross et al., 2007).

Continue reading "A "Follow the Energy" Approach for Astrobiology" »

Posted by Keith Cowing at 10:17 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 1006-1022

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2007.0156

Europa is a prime target for astrobiology. The presence of a global subsurface liquid water ocean and a composition likely to contain a suite of biogenic elements make it a compelling world in the search for a second origin of life. Critical to these factors, however, may be the availability of energy for biological processes on Europa.

Continue reading "Energy, Chemical Disequilibrium, and Geological Constraints on Europa" »

Posted by Keith Cowing at 9:47 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 971-986

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2006.0096

Dissolved H2 concentrations up to the mM range and H2 levels up to 9-58% by volume in the free gas phase are reported for groundwaters at sites in the Precambrian shields of Canada and Finland. Along with previously reported dissolved H2 concentrations up to 7.4 mM for groundwaters from the Witwatersrand Basin, South Africa, these findings indicate that deep Precambrian Shield fracture waters contain some of the highest levels of dissolved H2 ever reported and represent a potentially important energy-rich environment for subsurface microbial life. The

Continue reading "Hydrogeologic Controls on Episodic H2 Release from Precambrian Fractured Rocks--Energy for Deep Subsurface Life on Earth and Mars" »

Posted by Keith Cowing at 9:45 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 951-970

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2007.0150

Radiolysis of water may provide a continuous flux of an electron donor (molecular hydrogen) to subsurface microbial communities. We assessed the significance of this process in anoxic marine sediments by comparing calculated radiolytic H2 production rates to estimates of net (organic-fueled) respiration at several Ocean Drilling Program (ODP) Leg 201 sites. Radiolytic H2 yield calculations are based on abundances of radioactive elements (uranium, thorium, and potassium), porosity, grain density, and a model of water radiolysis. Net respiration estimates are based on fluxes of dissolved electron acceptors and their products. Comparison of radiolytic H2 yields and respiration at multiple sites suggests that radiolysis gains importance as an electron donor source as net respiration and organic carbon content decrease.

Continue reading "Radiolytic Hydrogen and Microbial Respiration in Subsurface Sediments" »

Posted by Keith Cowing at 9:44 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 933-950

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2006.0119

Numerical models are employed to investigate sources of chemical energy for autotrophic microbial metabolism that develop during mixing of oxidized seawater with strongly reduced fluids discharged from ultramafic-hosted hydrothermal systems on the seafloor. Hydrothermal fluids in these systems are highly enriched in H2 and CH4 as a result of alteration of ultramafic rocks (serpentinization) in the subsurface. Based on the availability of chemical energy sources, inferences are made about the likely metabolic diversity, relative abundance, and spatial distribution of microorganisms within ultramafic-hosted systems.

Continue reading "Geochemical Constraints on Sources of Metabolic Energy for Chemolithoautotrophy in Ultramafic-Hosted Deep-Sea Hydrothermal Systems" »

Posted by Keith Cowing at 9:43 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 891-904

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2006.0118

The common thread of energy release suggests that diverse microbial metabolic processes can be compared through thermodynamic analyses. The resulting energy and power requirements can provide quantitative constraints on habitability. Because previous thermodynamic analyses have focused on the minimum amount of energy needed for the growth of a microorganism or community, the focus of this study is to gain a fuller understanding of the microbial response to highly habitable conditions.

Continue reading "A Thermodynamic Analysis of Microbial Growth Experiments" »

Posted by Keith Cowing at 9:42 AM | Permalink | Comments (0)

Astrobiology December 2007, 7(6): 873-890

http://www.liebertonline.com/doi/pdfplus/10.1089/ast.2007.0127

Formate, a simple organic acid known to support chemotrophic hyperthermophiles, is found in hot springs of varying temperature and pH. However, it is not yet known how metabolic strategies that use formate could contribute to primary productivity in hydrothermal ecosystems. In an effort to provide a quantitative framework for assessing the role of formate metabolism, concentration data for dissolved formate and many other solutes in samples from Yellowstone hot springs were used, together with data for coexisting gas compositions, to evaluate the overall Gibbs energy for many reactions involving formate oxidation or reduction.

Continue reading "Formate as an Energy Source for Microbial Metabolism in Chemosynthetic Zones of Hydrothermal Ecosystems" »

Posted by Keith Cowing at 9:41 AM | Permalink | Comments (0)

A new paper in Nature from NAI's NASA Ames Research Center Team describes a new technique they've developed through which completely new enzymes can be evolved in the laboratory. The process does not require prior understanding of how the enzymes will work, or the use of product formation as a selection criterion.

Source: NAI Newsletter

Posted by Keith Cowing at 7:44 AM | Permalink | Comments (0)

The NAI Minority Institution Research Support Program is pleased to announce the selection of LeeAnne Martinez, an Associate Professor of Biology at Colorado State University - Pueblo, a Hispanic Serving Institution. Dr. Martinez plans to begin a genomic analysis of open water diatoms in the laboratory of Jim Lake, of the NAI UCLA team, to explore horizontal transfer of operational genes that may lead to the incorporation of endosymbionts by diatoms. LeeAnne's background includes nitrogen-fixation in diatom mats and this work will support current research at Colorado State-Pueblo. [Source: NAI Newsletter]

Posted by Keith Cowing at 12:12 PM | Permalink | Comments (0)

The Lab-On-a-Chip Application Development Portable Test System (LOCAD-PTS) is an instrument developed by the NAI Carnegie Institution of Washington Team over the past 4 years in collaboration with NASA Marshall Space Flight Center and Charles River Labs. LOCAD-PTS was flown to and recently tested aboard the International Space Station (ISS) to enable crew to monitor microorganisms and potentially hazardous chemicals within the cabin environment. The successful test is the first demonstration of this new technology, from sampling to data retrieval - by an astronaut in space. http://science.nasa.gov/headlines/y2007/06apr_locad2.htm?list123050 [Source: NAI Newsletter]

Posted by Keith Cowing at 10:53 PM | Permalink | Comments (0)

Mission Status Report: NASA's Orbiting GeneSat-1 Radios Date to Team on Earth

"The GeneSat-1 ground control station at NASA Ames will receive data radioed from the micro-laboratory after it has completed its observations and tests of the bacteria inside. The biological test will last only 96 hours, but the GeneSat-1 team will evaluate the stability of the orbiting payload's systems for four months to a year. The Small Spacecraft Office at NASA's Ames teamed up with industry and local universities to develop the fully automated, miniature GeneSat spaceflight system that provides life support for small living things."

GeneSat Mission Dashboard, Santa Clara University

GeneSat1, Real Time Satellite Tracking, NORAD ID: 29655 Int'l Code: 2006-058C

Where is GenSat1?

Posted by Keith Cowing at 1:06 AM | Permalink | Comments (0)

NASA's GeneSat-1 Reaches Orbit on Air Force Rocket, NASA

"NASA's GeneSat-1 rode an Air Force rocket into Earth orbit on Dec. 16, 2006 at 4 a.m. PST (7 a.m. EST) from NASA's Wallops Flight Facility, Wallops Island, Va. The satellite's locator beacon has been detected, and data has been received as GeneSat-1 orbits Earth, according to scientists."

View Launch Video

Posted by Keith Cowing at 10:19 AM | Permalink | Comments (0)

Bigelow Spacecraft Carries NASA 'GeneBox' for Tests in Orbit, NASA ARC

"On July 12, a Russian rocket lofted 'GeneBox' into Earth orbit within Bigelow Corporation's Genesis I test spacecraft. Attached to the large inflatable spacecraft's internal structure, GeneBox contains a miniature laboratory. In future flights, it will analyze how the near weightlessness of space affects genes in microscopic cells and other small life forms."

Posted by Keith Cowing at 12:09 AM | Permalink | Comments (0)